Clytin
WebJun 1, 2010 · The fluorescence quantum yield was determined to be 0.86, the brightness to be 54 mM−1 cm−1. For the first time we have also demonstrated an efficient radiationless energy transfer in vitro between clytin and cgreGFP in …
Clytin
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Webcretin. ( ˈkretin) noun. 1. a person who is mentally subnormal and physically deformed. cretino, que padece cretinismo. 2. an idiot, used as a term of contempt and abuse. … WebNov 17, 2009 · Here we present a spatial structure of a complex of the Ca (2+)-regulated photoprotein clytin with its green-fluorescent protein (cgGFP) from the jellyfish Clytia …
WebJul 1, 2007 · Clytin was cloned and sequenced from the jellyfish Clytia gregaria, and it shows 62% amino acid sequence homology to aequorin (32). Several isotypes are known: recombinant clytin-I showed lower... WebFeb 22, 2008 · Abstract. The cDNA for an isotype of clytin, a calcium-binding photoprotein from the luminous jellyfish Clytia gregarium, was identified and named clytin-II.The …
WebCloned GLP-1 receptor-expressing ChemiBrite cells were made by stable transfection of HEK293 cells with ChemiBrite clytin and the receptor and a promiscuous G protein to couple the receptor to the calcium signaling pathway. The cells have been cryopreserved at an optimal time post-transfection. WebClytin itself is not fluorescent, but the final product called Ca 2+ -discharged clytin, has a fluorescence maximum at 506 nm. Inclusion of GFP at µM concentrations shifts the bioluminescence spectrum to a 500 nm …
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WebApr 6, 2024 · The first His-Trp-Tyr (Phe instead of Tyr in obelin and clytin) triad surrounding the OH group of the 6-(p-hydroxyphenyl) substituent of the substrate was implied to be involved in the emitter formation and to account for the differences in the bioluminescence spectra of the hydromedusan photoproteins [5,26]. european countries starting with letter gWebFind 7 ways to say CRETIN, along with antonyms, related words, and example sentences at Thesaurus.com, the world's most trusted free thesaurus. european countries that offer free pre kWebJun 1, 2007 · Clytin-II (CL-II) is an isotype of the calcium-binding photoprotein clytin-I (CL-I) from Clytia gregaria. CL-II shows approximately 4.5-fold higher initial luminescence … european countries that allow gun ownershipWeb1 hour ago · Kershaw, who has enjoyed relative success against San Francisco, came into Wednesday’s match with a 1-1 record and a 3.75 ERA after two starts against the Diamondbacks. Immediately out of the ... european countries that have legalized weedWebJan 6, 2016 · The apparent dissociation constant of the clytin-cgreGFP complex was determined using the increase in bioluminescence intensity at λ = 500 nm upon addition … first aid in shockWebOct 1, 2024 · Both clytin and cgreGFP were separately crystallized and their spatial structures determined [6]. Ca2+-regu-lated photoprotein clytin is a globular protein with a diameter of ~ 45 A comprising tightly bound 2-hydro-peroxycoelenterazine in the center of its internal cavity [6]. The cgreGFP was crystallized as a homodimer [6] first aid inspection tagWebJun 1, 2007 · Clytin-II (CL-II) is an isotype of the calcium-binding photoprotein clytin-I (CL-I) from Clytia gregaria. CL-II shows approximately 4.5-fold higher initial luminescence intensity than CL-I with the same luminescence capacity, and is a potential candidate for a G-protein-coupled receptor assay among photoproteins. To investigate the high initial ... first aid in seizures