Cy3 channel wavelength
WebChannel: GL2: GL2: GL3: GL4: Compatible fluorescent proteins: tdTomato YFP: tmCherry : Antibody labels: Alexa Fluor 532 Alexa Fluor 555 DsRed Qdot 565 Qdot 605 R-phycoerythrin (R-PE, PE) Alexa Fluor 568 Alexa Fluor 594 Qdot 605 PE-Texas Red: Qdot 705 PE-Alexa Fluor 700 TRI-COLOR (PE-Cy5) PE-Cy5.5. Qdot 800 Web... key result was the observation of a signal in Image B (Cy5 channel) upon excitation at the Cy3 absorption wavelength, with a control study indicating no emission …
Cy3 channel wavelength
Did you know?
WebATTO-TEC GmbH Martinshardt 7 D-57074 Siegen Germany Phone: +49 271 23853 – 0 FAX: +49 271 23853 – 11 E-mail: [email protected] http: www.atto-tec.com Revised: 2024-12-13 ATTO 550 is a novel fluorescent label related to the well-known dye Rhodamine 6G. The dye is designed for application in the area of life science, e.g. labeling of Because they yield brighter and more stable fluorescence, cyanines can advantageously replace conventional dyes such as fluorescein and rhodamines. • Cy3 and Cy5 are the most popular, used typically combined for 2 colors detection. Cy3 fluoresces greenish yellow (~550 nm excitation, ~570 nm emission), while …
WebCy3 can be excited to about 50% of maximum with an argon laser (514 nm or 528 nm lines), or to about 75% of maximum with a helium/neon laser (543 nm line) or mercury lamp (546 nm line). Cy3 is commonly combined with green-fluorescent dyes for double labeling. However, due to the spectral overlap the use of a narrow band-pass emission filter for ... WebDec 1, 2001 · Cy3. Manual, six position slider for fluorescence filters. Special optical system for epi-illumination microscopy with wavelength switching using a polychroic …
WebJan 10, 2024 · Fluorescent dyes and their excitation and emission wavelength peaks The table below shows a comprehensive list of fluorescent dyes with their respective … Webemission wavelengths. Get more information on dye spectral emission and excitation values. Choose dyes that can be calibrated on your instrument. Your real-time PCR instrument must be calibrated for the set of dyes you choose (Table 1). If it is not, you must calibrate the instrument or select different dyes. IDT provides
WebUnlike a static image showing excitation and emission histograms, this tool will demonstrate how a fluorochrome will have the same emission profile—but have varying brightness—based on the excitation wavelength of the laser. Choosing different laser wavelengths will transform the emission curve based on the percent excitation at that …
WebJul 1, 2024 · What color is Cy3? Posted . July 1, 2024. Bioconjugation. Classic Dyes. Cynines. Answer. Cy3 is orange in color. It fluoresces greenish yellow at 555 nm … physik potential definitionWebCy3 dye. Fluorescence SpectraViewer. Invitrogen Cy3 dye is a bright, orange-fluorescent dye that can be excited using the 532 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. In addition to immunocytochemistry applications, Cy3 … Invitrogen Cy5 dye is a bright, far-red-fluorescent dye with excitation ideally … physik newtonWebLeading Life Science Research & Clinical Diagnostics — Bio-Rad physik pearsonWebTYE 563 can be used as a direct substitute for Cy3®. View product details for TYE™ 563: 5' 3' ... A red wavelength dye commonly used in multiplex reactions with FAM or in fluorescence-activated cell sorting (FACS) and microscopy applications using the rhodamine filter. TEX 615 can be used as a direct substitute for Texas Red or CAL Fluor 610. physik oberstufe bayernWebInvitrogen Alexa Fluor 568 dye is a bright, orange-fluorescent dye with excitation ideally suited for the 568 nm laser line on the Ar-Kr mixed-gas laser. For stable signal generation in imaging and flow cytometry, Alexa Fluor 568 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability ... physiko therapie triester straße 9WebJun 18, 2024 · Choose dyes with appropriate excitation wavelengths and little to no overlap in their emission spectra. Consider total fluorescence intensity as well. For example, … physik mondphasenWebwavelength (nm) wavelength (nm) lines (nm) DAPI 359 461 325,360,405,407 Hoechst 33258 352 461 Hoechst 33342 350 461 SYTOX Blue 431 480 YOYO-1 491 509 SYTOX … physikolympiade 2021 thüringen