WebJun 1, 2024 · 2.2. Immunofluorescence (IF) IF was performed on paraffin embedded placental sections as previously performed in our laboratory [20].Briefly, serial sections were incubated overnight with rabbit polyclonal antibodies against phospho (p) AKT, pERK, pmTOR, pp70, p4EBP1(all from cell Signaling, Danvers, MA) or cytokeratin 7 (for …
Insulin-stimulated translocation of GLUT4 to the plasma …
WebApr 11, 2024 · Double immunofluorescence. ... AICAR effect on inhibition of cell growth and migration, it was found that the activation of AMPK led to decreased pAKT (Thr308) through inactivation of PI3K. Interestingly, pAKT (Ser473) levels were significantly increased in AICAR treated cells which may be due to the fact that rictor-mTOR complex regulates … WebThis protocol describes Multiplex IHC staining using IF detection. Download a pdf version of the the IHC-IF Multiplex protocol. Download Multiplex immunofluorescence allows the simultaneous detection of several target proteins in the same cell. how rna vaccines work video
Hippocampal GSK3β as a Molecular Link Between Obesity and Depression
WebApr 9, 2011 · Immunofluorescence Confocal Analysis CRC cells grown on glass coverslips were exposed to the indicated concentrations of gefitinib, AG1024, or both. After incubation for 3 h, covered cells were rinsed with PBS, fixed in 4% paraformaldehyde at room temperature for 20 min, and then permeabilized with ice-cold 2.5% Triton for 3–5 min. WebOct 9, 2014 · To investigate if Mapk/Erk and PI3K/Akt signaling are also restricted to MG-derived progenitors, we investigated pErk and pAkt expression in the uninjured and injured retina using immunofluorescence. Interestingly, pErk and pAkt increased in MG and photoreceptor outer segments, respectively, throughout the retina within 1 hpi (Figure S3 … WebThe densitometry analysis of pAkt immunofluorescence staining, of BM MKs, is derived from 4 mice per group. At least 30 MKs per section were analyzed. The flow cytometry analysis of pAkt phosphorylation was performed in CD41 + BM MKs. The mean fluorescence intensity (MFI) is shown. Data are expressed as mean±standard deviation … how road roller drive