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Pakt immunofluorescence

WebJun 1, 2024 · 2.2. Immunofluorescence (IF) IF was performed on paraffin embedded placental sections as previously performed in our laboratory [20].Briefly, serial sections were incubated overnight with rabbit polyclonal antibodies against phospho (p) AKT, pERK, pmTOR, pp70, p4EBP1(all from cell Signaling, Danvers, MA) or cytokeratin 7 (for …

Insulin-stimulated translocation of GLUT4 to the plasma …

WebApr 11, 2024 · Double immunofluorescence. ... AICAR effect on inhibition of cell growth and migration, it was found that the activation of AMPK led to decreased pAKT (Thr308) through inactivation of PI3K. Interestingly, pAKT (Ser473) levels were significantly increased in AICAR treated cells which may be due to the fact that rictor-mTOR complex regulates … WebThis protocol describes Multiplex IHC staining using IF detection. Download a pdf version of the the IHC-IF Multiplex protocol. Download Multiplex immunofluorescence allows the simultaneous detection of several target proteins in the same cell. how rna vaccines work video https://smallvilletravel.com

Hippocampal GSK3β as a Molecular Link Between Obesity and Depression

WebApr 9, 2011 · Immunofluorescence Confocal Analysis CRC cells grown on glass coverslips were exposed to the indicated concentrations of gefitinib, AG1024, or both. After incubation for 3 h, covered cells were rinsed with PBS, fixed in 4% paraformaldehyde at room temperature for 20 min, and then permeabilized with ice-cold 2.5% Triton for 3–5 min. WebOct 9, 2014 · To investigate if Mapk/Erk and PI3K/Akt signaling are also restricted to MG-derived progenitors, we investigated pErk and pAkt expression in the uninjured and injured retina using immunofluorescence. Interestingly, pErk and pAkt increased in MG and photoreceptor outer segments, respectively, throughout the retina within 1 hpi (Figure S3 … WebThe densitometry analysis of pAkt immunofluorescence staining, of BM MKs, is derived from 4 mice per group. At least 30 MKs per section were analyzed. The flow cytometry analysis of pAkt phosphorylation was performed in CD41 + BM MKs. The mean fluorescence intensity (MFI) is shown. Data are expressed as mean±standard deviation … how road roller drive

Differential expression of mTOR related molecules in

Category:pAKT (Phosphorylated AKT) NeoGenomics Laboratories

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Pakt immunofluorescence

Immunofluorescence Staining of Paraffin Sections Step by Step

WebApr 11, 2024 · Immunofluorescence Cells were fixed in pre-chilled 4% paraformaldehyde for 15 min, treated with 0.5% Triton for 5 min, blocked with 10% goat serum at 4°C for 60 min, and incubated with primary antibodies at 4°C overnight. WebNov 9, 2024 · Immunofluorescence staining is the most frequently applied technique to detect and visualize various molecules in biological samples. Many protocols can be found in the literature and the websites of commercial antibody producers. This can result in a time-consuming and costly methodical work to est …

Pakt immunofluorescence

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WebAug 29, 2014 · h–k pAkt immunofluorescence in the dentate gyrus of rats fed a HF diet after stimulation of brain slices with 5-HT (10 μM), leptin (10 nM) and insulin (100 nM) for 5 min. Corresponding DAPI-counterstained sections (blue) are shown on the right top corner of each picture. Confocal laser scanning. Focal planes. WebImmunofluorescence analysis for p-Akt (Ser473) and Cleaved Caspase-3. (A) The expression and location of p-Akt (Ser473) in UMUC3 cells treated with 0 or 20 μM GLA …

WebNational Center for Biotechnology Information WebAug 11, 2009 · Immunofluorescence for pAkt was very low in rats given icv saline (red fluorescence, Panel B); conversely, pAkt immunoreactivity was detected in the CA3 region 30 minutes following icv administration of insulin (Panel E). Insulin-stimulated phosphorylation of Akt was blocked by pre-treatment with LY-294002 (Panel H). The …

WebQuantitative proteomics analysis confirmed that the protein cargo of EVs are modified upon activation of PI3K/Akt signaling pathway. Bioinformatics analysis highlighted the … WebApr 14, 2014 · Immunofluorescence analysis of Dicer morphants revealed dramatically reduced pAkt and pS6 staining in the pronephric kidney ( Fig. 4 A – D ′ ). This suggested that mTORC1 signaling is impaired in the absence of mature miRNAs.

WebWe further validated our findings from RNA-ISH staining by immunofluorescence (IF) (Figure S1 G). In keeping with our RNA-ISH data, LY6D + cells were readily detectable in PTEN-deficient cells, identified by positive staining for YFP and phosphorylated alpha serine/threonine protein kinase (pAKT) in HN and CR prostates ( Figure 1 F).

WebSep 16, 2011 · Immunofluorescence of Germ Cells via Anti-Kcr Antibody. Tubules of mice were prepared as previous reported (Kotaja et al., 2004). Cells were fixed in 90% ethanol for 10 min at room temperature. They were permeabilized with 0.2% Triton X-100 and 0.5% saponine in PBS for 15 min at room temperature and blocked with 5% milk in PBS. They … merrick flower shoppeWebSep 1, 2008 · In the immunofluorescence analysis, the cell lines studied (AP4 and M1) showed nuclear reactivity to Mdm2 and pAkt. The p53 and p21 expression were … merrick flower shoppe merrick nyWebMar 1, 2024 · For pAKT immunofluorescence, overnight fasted animals were administered Actrapid (2 mU/kg, Novo Nordisk, Bagsværd, Denmark) intravenously. Animals were sacrificed 15 min after administration and perfused as described before. 4.5. Immunohistochemistry for POMC-eGFP, pStat3 and pAkt. merrick foodWebpAKT (Phosphorylated AKT) Methodology Immunohistochemistry (IHC) Test Description AKT is a signal transduction protein that plays a central role in inhibiting apoptosis in a … how ro add minecraft forge 1.10.2WebThe DNA dye DAPI in combination with immunofluorescence and confocal microscopy were used to identify the nucleated cells (blue fluorescence) that contained pAkt (red fluorescence) in... merrick food ratedWebIn parallel with RAD001-induced inhibition of cell viability, a dose- and schedule- dependent down-regulation of pAKT and p-eIF4E expressions was demonstrated. In contrast, a … merrick food shelfWebImmunogen Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser473 of human Akt. Validations … merrick food reviews