The sybr premix ex taq kit
Web李秋鸿，黄清松，毛建文 (广东药科大学生命科学与生物制药学院/广东省生物活性药物研究重点实验室，广东 广州 510006) WebDownload scientific diagram The effect of circSEPT9 silencing on tumor formation in vivo. (a-c) The effect of circSEPT9 knockdown on tumor formation. (d and e) CircSEPT9, miR-149-5p and SLC1A5 ...
The sybr premix ex taq kit
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WebDownload scientific diagram GPNA treatment inhibited MDA-MB-231 and BT-549 cell processes. (a-c) SLC1A5 expression at mRNA and protein level was detected by qRT-PCR and Western blot analysis ... WebThe transcriptional levels of NF-κB p65, IKKβ, IL-6, IL-1β, and TNF-α were detected by RT-qPCR using the SYBR Green chimeric fluorescence method with SYBR Premix Ex Taq (Perfect Real Time) Kit reagent. The comparative 2−ΔΔCt value quantitative method was used for relative quantification.
WebFinally, RT-qPCR was carried out for 40 cycles (95°C for 5 seconds and 60°C for 34 seconds) using SYBR™ Premix Ex Taq kit on an 7500 RT-PCR Instrument (Thermo Fisher Scientific). The relative expression of p53 gene was calculated and normalized by 2 −ΔΔCT method, using β-actin as a reference gene. The primers were used as follows: Webwith the SYBR Premix Ex Taq (Takara, Japan) in a total volume of 20L. Each reaction mixture contained template DNA, 10L of 2× SYBR Premix Ex Taq, and 0.1M of each primer. Standard curves were obtained using serial dilutions of a known copy number of plasmid containing a 16S rRNA gene fragment, and these were lin-
WebArticle Snippet: The collected cDNA was used for RT–qPCR SYBR premix Ex Taq II Kit (Takara Biotechnology) in a Bio-Rad CFX96. Techniques: Expressing, Luciferase, … WebTB Green Premix Ex Taq II (Tli RNaseH Plus) is a TB Green Master Mix that effectively suppresses amplification of primer dimers and off-target products, and is therefore …
WebCat. # Product Size License Quantity Details; RR39WR Premix Ex Taq™ (Probe qPCR), ROX Plus: 1,000 Rxns: USD $1246.00: Premix Ex Taq (Probe qPCR) is a 2X premix for real-time …
WebInvitrogen™SuperScript™ III Platinum™ SYBR™ Green One-Step qPCR Kit w/ROX quantity. Add to cart. SKU: 11746500 Category: Real Time PCR Reagents and kits. ... SYBR: Polymerase: Taq DNA Polymerase: For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT System: Product Type: oxford\u0027s zero emission zoneイポー 観光 マップWebApr 14, 2024 · RT-qPCR was executed through SYBR Premix Ex Taq kit (Takara, China) together with Step-one real-time PCR system (ABI, United States). The mRNA level was standardized to Tubulin. The primers were synthesized by Sangon (China) . Relative mRNA level was computed based upon 2 −ΔΔCT approach. TABLE 1 ... oxford to penzanceWeb本制品对 SYBR Green I 浓度、PCR 反应体系都进行了优化，采用了反应性能优越的 Pro Taq HS DNA polymerase 体系，能够有效抑制非特异性产物的扩增，提高 PCR 扩增效率，可以进行高灵敏度的 Real Time PCR 反应，在宽广的定量区域内得到良好的标准曲线，从而对靶基因 … oxford uni casual pay spineWebSep 13, 2024 · The DNA sample extracted in Example 1 was used as a template. according to The Premix Ex Taq TM kit instructions operate, add the reagents required for real-time quantitative PCR, ... SYBR Green fluorescent quantitative RT-PCR primer for detecting Nebovirus and application CN112126697A (en) イボイノシシ マングースWebMar 1, 2024 · Premix Ex Taq是一种2X 浓度的Premix Type试剂，进行实验时，PCR反应液的配制十分方便简单。. Mix中添加了Tli RNaseH (耐热性RNaseH)，以cDNA作为模板进 … oxford to silverstone circuitWebApr 10, 2024 · RT reagent Kit with gDNA Eraser (TaKaRa). For the nested PCR, the primers targeted on the N gene of BJNV were designed based on the results of viral metagenomics (Table S1). The 25?L first-round PCR mixture included 2.5?L 10 PCR reaction buffer, 50?mM MgCl2, 5?pmol of each primer, 1?L cDNA 0.5?mM dNTP, and 0.1?L ExTaq Enzyme (Takara). oxford travel zones